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Analyze Cells with Park Scanning Probe Microscopy

Brian Choi, (This email address is being protected from spambots. You need JavaScript enabled to view it.) Bio-application scientist

To study cell at fundamental level, cell’s physical properties (morphology and stiffness/elasticity/adhesion force) must be measured accurately.

 

quantitative-mechanical-property-imaging-by-PinPoint-modes

 
  • Analyze cell at fundamental level, by acquiring cell morphology and quantitative mechanical property image of cell simultaneously
  • PinPoint™ mode allows users to visualize elasticity (Young’s modulus) and adhesion force as an image providing quantitatively calculated values

 

 

PinPoint mode™ is a newly developed AFM imaging mode that acquires topographical data and the FD data at each pixel simultaneously. Using PinPoint™ mode, cell morphology and the quantitative nanomechanical properties of cell can be obtained at the same time. The height information constituting the morphology of the sample surface is determined when the designated stiffness threshold is reached. The elasticity (stiffness) at certain positions of the sample surface can be obtained the same way as in the FD spectroscopy. The stiffness can be obtained from the slope of FD curve from contact point to deflection threshold generated during the PinPoint mode™ operation. Applying Hertz model, designed for soft and elastic samples, the user can calculate the elasticity (Young's modulus) from the slope, too. The adhesion image is generated based on the maximum adhesion force value.

pinpoint-mode-control PinPoint™ mode Control
 

Cell Mechanical Property Study with PinPoint™ mode

cell-mechanical-property-study-with-pinpoint-mode

 

Park Cell Analysis Systems

  park-analyze-cell-nx-bio park-analyze-cell-nx10 park-analyze-cell-xe7
  Park NX-Bio Park NX10 Park XE7
Scanning Ion Conductance Microscopy (SICM)  
Atomic Force Microscopy (AFM) with liquid probe hand
Inverted Optical Microscopy (IOM)    
Live Cell Chamber    
 

Analyze Cells - AFM | Atomic Force Microscopy |Park Systems