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    NX-Bio
    SICM and AFM Options
 

Hardware Options

live-cell-chamber

Live Cell Chamber

活细胞室为细胞创造了理想的生存环境,通过控制最理想的温度、pH和湿度,延长细胞的寿命,使其在长时间的测量中保持活性。活细胞室试验表明细胞能够存活20个小时以上。

live-cell
 
ICM-image-of-Rat

Ion Channel Recording of Targeted Patch Clamping

Conventional Patch Clamping is an optical microscope view-based technique used to monitor a single living cell's ion channel activity—a key quantifier of various cellular activities. Targeted Patch Clamping is the SICM-based version of this technique that enables the detection of ion channel activities of specific subcellular structures.

SICM + Patch Clamping = Targeted Patch Clamping

Specified-Ion-Channel-Activity-Study
 

More Comprehensive Cell Biology Study, by Integrating Fluorescence Microcopy with Park SICM

Combining fluorescence microscopy (FM) techniques with Park SICM can create new benefits and provide comprehensive information for cell biology studies that cannot be obtained when using only one of those techniques. While monitoring external cellular surface morphology with SICM, the internal cellular behavior can be observed by FM.

Integrating-Fluorescence-Microcopy
 

Structural & Physical Property Characterization of Cell in XEI

Structural-n-Physical-Property-Characterization-of-Cell-in-XEI
 

Software Option

SmartScanTM – Data Acquisition

A原子力显微镜测量中的所有用户控制均通过SmartScanTM数据获取程序实现。 以用户为导向的界面可使原子力显微镜操作简便。

• 同时采集多达16个成像数据。
• 最大4096 × 4096的成像尺寸
• 具有批处理功能的专用力距和电流-电压光谱
• 悬臂梁弹簧常数校准 (选项)
• 通过外部软件,脚本级控制 (LabVIEW, C++)

 

XEI – Image Processing and Analysis

XEI是原子力显微镜图像处理和分析程序。其强大的处理演算法使分析变得更容易且效率更高。 由于XEI具有最先进且多种成像特征,NX用户可从实验中获得必须的关键信息。

• 谱线开关、区域和三维渲染的图像分析
• 光谱数据分析模块(F-d、I-V)
• 直接复制/粘贴到显示程序
• Multiple image comparison
• 多种图像比较
两个不同的图像重叠

 

Image Overlay: SICM Topography + Fluorescence Microscopic Image

nx-bio-sicm-overlay

借助图像合成技术,倒置光学显微镜的光学数据可与原子力显微镜获取的形貌数据相合成。该功能让用户可以创建完整的四维表面。在上图中,XE-Bio所拍摄的珠状聚二甲基硅氧烷的三维形貌图与倒置光学显微镜的荧光数据合成,构成第四维。

 

Park NX-Bio - Options | Park Atomic Force Microscope | Scanning Ion Conductance Microscopy